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human lgg cell lines hs683 (ATCC)

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ATCC human lgg cell lines hs683

Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in <t>HS683</t> and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Human Lgg Cell Lines Hs683, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lgg+cell+lines+hs683/Hs+683/pmc13217492-249-1-22
Average 95 stars, based on 337 article reviews

human lgg cell lines hs683 - by Bioz Stars, 2026-09

95/100 stars

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1) Product Images from "Integrating multi-omics analysis and machine learning to refine molecular subtypes and prognostic assessment of lower-grade glioma"

Article Title: Integrating multi-omics analysis and machine learning to refine molecular subtypes and prognostic assessment of lower-grade glioma

Journal: Molecular Therapy Oncology

doi: 10.1016/j.omton.2026.201227

Figure Legend Snippet: Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in HS683 and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Techniques Used: Expressing, Functional Assay, Biomarker Discovery, Western Blot, Knockdown, Migration, Wound Healing Assay, Cell Counting, Flow Cytometry

other:

Article Title: Development of a metabolic subtype classifier for low-grade glioma to guide precision therapy.
Article Snippet: Human astroglial cells (SVG p12, Cell Research, ZQ1160) and LGG cell lines Hs683 (Cell Research, ZQ0049) and SW1088 (Procell, CL-0948) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

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Journal: Molecular Therapy Oncology

Article Title: Integrating multi-omics analysis and machine learning to refine molecular subtypes and prognostic assessment of lower-grade glioma

doi: 10.1016/j.omton.2026.201227

Figure Lengend Snippet: Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in HS683 and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Article Snippet: The human LGG cell lines HS683 and H4, as well as the human embryonic kidney cell line HEK293T, were purchased from the American Type Culture Collection (ATCC).

Techniques: Expressing, Functional Assay, Biomarker Discovery, Western Blot, Knockdown, Migration, Wound Healing Assay, Cell Counting, Flow Cytometry

Colony formation abilities of T98G and Hs683 cells treated with CVB-D. CVB-D, Cyclovirobuxine D.

Journal: Oncology Reports

Article Title: Cyclovirobuxine D inhibits cell proliferation and migration and induces apoptosis in human glioblastoma multiforme and low-grade glioma

doi: 10.3892/or.2020.7459

Figure Lengend Snippet: Colony formation abilities of T98G and Hs683 cells treated with CVB-D. CVB-D, Cyclovirobuxine D.

Article Snippet: The GBM cell line T98G and the LGG cell line Hs683 were obtained from ATCC.

Techniques:

CVB-D reduces cell viability of T98G and Hs683 cells. Each experiment involved three replicates. CVB-D, Cyclovirobuxine D.

Journal: Oncology Reports

Article Title: Cyclovirobuxine D inhibits cell proliferation and migration and induces apoptosis in human glioblastoma multiforme and low-grade glioma

doi: 10.3892/or.2020.7459

Figure Lengend Snippet: CVB-D reduces cell viability of T98G and Hs683 cells. Each experiment involved three replicates. CVB-D, Cyclovirobuxine D.

Article Snippet: The GBM cell line T98G and the LGG cell line Hs683 were obtained from ATCC.

Techniques:

Cell cycle of CVB-D-treated glioma cells. (A) Cell cycle analysis of CVB-D treated T98G and Hs683 cells as detected by flow cytometry (0, 80, 160 and 240 µmol/l). (B) Statistical analysis of the percentage of cells at the G 0 /G 1 , S and G 2 /M phases of the T98G and Hs683 cell cycle. Each group was compared with the 0 µmol/l group with regard to the S phase. **P<0.01, ***P<0.001, ****P<0.0001. CVB-D, Cyclovirobuxine D.

Journal: Oncology Reports

Article Title: Cyclovirobuxine D inhibits cell proliferation and migration and induces apoptosis in human glioblastoma multiforme and low-grade glioma

doi: 10.3892/or.2020.7459

Figure Lengend Snippet: Cell cycle of CVB-D-treated glioma cells. (A) Cell cycle analysis of CVB-D treated T98G and Hs683 cells as detected by flow cytometry (0, 80, 160 and 240 µmol/l). (B) Statistical analysis of the percentage of cells at the G 0 /G 1 , S and G 2 /M phases of the T98G and Hs683 cell cycle. Each group was compared with the 0 µmol/l group with regard to the S phase. **P<0.01, ***P<0.001, ****P<0.0001. CVB-D, Cyclovirobuxine D.

Article Snippet: The GBM cell line T98G and the LGG cell line Hs683 were obtained from ATCC.

Techniques: Cell Cycle Assay, Flow Cytometry

CVB-D induces apoptosis of T98G and Hs683 cells. (A) Apoptotic analysis was performed by flow cytometry. (B) Statistical analysis of the percentages of apoptotic cells following treatment with various concentrations of CVB-D. Each treatment dosing group was compared with the control group. *P<0.05, ***P<0.001, ****P<0.0001. Survival: PI − /Annexin V − ; early apoptosis: PI − /Annexin V + ; late apoptosis: PI + /Annexin V + . Each experiment involved three replicates. CVB-D, Cyclovirobuxine D.

Journal: Oncology Reports

Article Title: Cyclovirobuxine D inhibits cell proliferation and migration and induces apoptosis in human glioblastoma multiforme and low-grade glioma

doi: 10.3892/or.2020.7459

Figure Lengend Snippet: CVB-D induces apoptosis of T98G and Hs683 cells. (A) Apoptotic analysis was performed by flow cytometry. (B) Statistical analysis of the percentages of apoptotic cells following treatment with various concentrations of CVB-D. Each treatment dosing group was compared with the control group. *P<0.05, ***P<0.001, ****P<0.0001. Survival: PI − /Annexin V − ; early apoptosis: PI − /Annexin V + ; late apoptosis: PI + /Annexin V + . Each experiment involved three replicates. CVB-D, Cyclovirobuxine D.

Article Snippet: The GBM cell line T98G and the LGG cell line Hs683 were obtained from ATCC.

Techniques: Flow Cytometry, Control

Hoechst staining is used to observe the morphological characteristics of T98G and Hs683 cells. The results indicated that the percentage of hyperchromatic nuclei was increased as the concentration of CVB-D increased. The MFI was assessed by Image-Pro Plus 6.0, and statistically analyzed by GraphPad Prism 8. *P<0.05. CVB-D, Cyclovirobuxine D; MFI, mean fluorescence intensity.

Journal: Oncology Reports

Article Title: Cyclovirobuxine D inhibits cell proliferation and migration and induces apoptosis in human glioblastoma multiforme and low-grade glioma

doi: 10.3892/or.2020.7459

Figure Lengend Snippet: Hoechst staining is used to observe the morphological characteristics of T98G and Hs683 cells. The results indicated that the percentage of hyperchromatic nuclei was increased as the concentration of CVB-D increased. The MFI was assessed by Image-Pro Plus 6.0, and statistically analyzed by GraphPad Prism 8. *P<0.05. CVB-D, Cyclovirobuxine D; MFI, mean fluorescence intensity.

Article Snippet: The GBM cell line T98G and the LGG cell line Hs683 were obtained from ATCC.

Techniques: Staining, Concentration Assay, Fluorescence

Rh123 staining of T98G and Hs683 cells following various concentrations of CVB-D pretreatment. The staining analysis was performed by flow cytometry. The results indicated that the hyperchromatic cells were increased as the concentration of CVB-D increased. The change in low staining of FITC + cells corresponded to the MMP change of the cells (MMP or ΔΨm). Statistical analysis of low staining of FITC + cells was performed using SPSS 22. *P<0.05. CVB-D, Cyclovirobuxine D; Rh123, Rhodamine 123.

Journal: Oncology Reports

Article Title: Cyclovirobuxine D inhibits cell proliferation and migration and induces apoptosis in human glioblastoma multiforme and low-grade glioma

doi: 10.3892/or.2020.7459

Figure Lengend Snippet: Rh123 staining of T98G and Hs683 cells following various concentrations of CVB-D pretreatment. The staining analysis was performed by flow cytometry. The results indicated that the hyperchromatic cells were increased as the concentration of CVB-D increased. The change in low staining of FITC + cells corresponded to the MMP change of the cells (MMP or ΔΨm). Statistical analysis of low staining of FITC + cells was performed using SPSS 22. *P<0.05. CVB-D, Cyclovirobuxine D; Rh123, Rhodamine 123.

Article Snippet: The GBM cell line T98G and the LGG cell line Hs683 were obtained from ATCC.

Techniques: Staining, Flow Cytometry, Concentration Assay

Scratch test of T98G and Hs683 cells. The scratch test was conducted at 12 h and the results indicated that tumor cell migration was significantly inhibited following a concentration increase of CVB-D. Distances between the edges of the cells was analyzed by SPSS 22. *P<0.05. CVB-D, Cyclovirobuxine D.

Journal: Oncology Reports

Article Title: Cyclovirobuxine D inhibits cell proliferation and migration and induces apoptosis in human glioblastoma multiforme and low-grade glioma

doi: 10.3892/or.2020.7459

Figure Lengend Snippet: Scratch test of T98G and Hs683 cells. The scratch test was conducted at 12 h and the results indicated that tumor cell migration was significantly inhibited following a concentration increase of CVB-D. Distances between the edges of the cells was analyzed by SPSS 22. *P<0.05. CVB-D, Cyclovirobuxine D.

Article Snippet: The GBM cell line T98G and the LGG cell line Hs683 were obtained from ATCC.

Techniques: Migration, Concentration Assay

Various concentrations of CVB-D induce the expression of apoptosis-associated proteins in T98G and Hs683 cells. The cells were pretreated with various concentrations of CVB-D for 6 h and the protein bands were detected by western blotting. β-actin was used as the control protein. The software ImageJ (version 1.50i) was used for densitometric analysis of the protein expression and statistical analysis was performed using SPSS 22 software. *P<0.05. CVB-D, Cyclovirobuxine D.

Journal: Oncology Reports

Article Title: Cyclovirobuxine D inhibits cell proliferation and migration and induces apoptosis in human glioblastoma multiforme and low-grade glioma

doi: 10.3892/or.2020.7459

Figure Lengend Snippet: Various concentrations of CVB-D induce the expression of apoptosis-associated proteins in T98G and Hs683 cells. The cells were pretreated with various concentrations of CVB-D for 6 h and the protein bands were detected by western blotting. β-actin was used as the control protein. The software ImageJ (version 1.50i) was used for densitometric analysis of the protein expression and statistical analysis was performed using SPSS 22 software. *P<0.05. CVB-D, Cyclovirobuxine D.

Article Snippet: The GBM cell line T98G and the LGG cell line Hs683 were obtained from ATCC.

Techniques: Expressing, Western Blot, Control, Software

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human lgg cell lines hs683 (ATCC)

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1) Product Images from "Integrating multi-omics analysis and machine learning to refine molecular subtypes and prognostic assessment of lower-grade glioma"

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